Cycle 1 mid-length ^^^ragmenj^template^
Cycle 1 mid-length fragment (template)
Cycle 2 short fragment (template)
Short fragment Short fragment
^Cycle 2 short fragment (template)
Figure 9.24 The Final PCR Product Is a Fragment of Discrete Size The positions to which the primers anneal to the original template molecule dictate the size and sequence of the fragment that is amplified exponentially.
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