Cycle 1 mid-length ^^^ragmenj^template^


Short fragment

^Short fragment

Cycle 1 mid-length fragment (template)

Cycle 2 short fragment (template)

Short fragment Short fragment

^Cycle 2 short fragment (template)

Figure 9.24 The Final PCR Product Is a Fragment of Discrete Size The positions to which the primers anneal to the original template molecule dictate the size and sequence of the fragment that is amplified exponentially.

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