Cultivating Prokaryotes in the Laboratory

By knowing environmental and nutritional factors that influence growth of specific prokaryotes, it is often possible to provide appropriate conditions for their cultivation. These include a medium on which to grow the organisms and a suitable atmosphere.

General Categories of Culture Media

Considering the diversity of bacteria, it is not surprising that a wide variety of media is used to culture them. For routine pur poses, one of the many types of complex media is used. In contrast, chemically defined media are generally used for specific research experiments when nutrients must be precisely controlled. Table 4.5 summarizes the characteristics of various types of media.

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