B

7f/i vl fragments

Isolate DNA

Digest with restriction enzyme

Separate DNA fragments using gel electrophoresis

Denature DNA then transfer DNA to filter

Add single-stranded labeled probe

Detect probe

Figure 9.8 The Steps of a Southern Blot This technique is used to verify the presence or absence of a given sequence and determine the size of any restriction enzyme fragments that contain the sequence. If the DNA fragments contain the same genes, why are the fragments different in length?

those particular culture conditions. By repeating the experiment using a culture grown under a different set of conditions, perhaps an environment that mimics what a disease-causing microbe would encounter in the body, differences in gene expression can be demonstrated.

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