A 5s B 16s

C. 23S D. All of these are commonly used. 10. Which of the following statements is false?

A. Tropheryma whippelii could be identified before it had been grown in culture.

Review Questions

B. The GC content of DNA can be measured by determining the temperature at which double-stranded DNA melts.

C. Sequence differences between organisms can be viewed as an evolutionary chronometer to assess their relatedness.

D. Based on DNA homology studies, members of the genus Shigella should be in the same species as Escherichia coli.

E. Pulsed-field gel electrophoresis is used to determine the ribo-type of an organism.

Applications

1. Microbiologists debate the use of biochemical similarities and cell features as a way of determining the taxonomic relationships among prokaryotes. Explain why some microbiologists believe these similarities and differences are a powerful taxonomic indicator while others think it is not very useful for that purpose.

2. The mitochondria of eukaryotic cells are theorized to have bacterial origins. A researcher interested in investigating the relationship of mitochondria to bacteria must now decide on the best method to make this determination. What advice would you give the researcher?

Critical Thinking

1. In figure 10.16, how would the curve appear if the GC content of the DNA sample were increased? How would the curve appear if the AT content were increased?

2. When DNA probes are used to detect specific sequence similarities in bacterial DNA, the probe is heated and the two strands of DNA are separated. Why must the probe DNA be treated in this way before detection is possible?

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