Methanol is determined by gas chromatography. The clinical diagnosis of methanol poisoning is difficult in the absence of a history of ingestion. The objective signs of ocular toxicity of methanol include dilated pupils, which are partially reactive or non-reactive to light, and optic disk hyperemia with blurring of the disk margins.

If a specific analytical method for methanol is not available, the anion and osmolal gaps should be calculated. The anion gap (AG) can be calculated using the formula

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