ALPF Medical Research

» Cardiac Hypertrophy

  • Acknowledgments
  • Amplification Method The Double Plasmid System
  • Animal Models
  • Cell Culture
  • Cloning
  • Congenital Heart Disease
  • CreER and CrePRInducible Systems
  • CreoxP in the Heart
  • Delivery of the AdnNOS Vector or ODNs Into the PVN by Microinjection
  • Delivery of the AdnNOS Vector or ODNs Into the PVN by Microinjection see Note
  • Determination of nNOS Expression Within the PVN
  • Discussion
  • Dual Modality Reporter Imaging Technology
  • E coli Transformation and Selection
  • End Point Dilution Assay
  • Expression of AdvheNOS in HAAE1 Cells
  • Fluorescent Protein Reporter Gene
  • Functional Study Effect of Manipulation of nNOS Within the PVN on Sympathetic Nerve Activity Blood Pressure and Heart Rate
  • HeNOS Activity Assay
  • Human eNOS Full Length cDNA
  • Inducible Gene Expression Systems 31 Introduction
  • Info
  • Inhibition of nNOS by Antisense
  • Introduction - 2 3 4 5 6
  • Mark Jones and Walter J Koch Summary
  • Lentivirus Vector Gene Delivery Systems
  • Luciferase Reporter Gene and Bioluminescence Imaging
  • Materials - 2 3
  • Measurement of NO Production and heNOS Activity
  • Measurement of NOx
  • Merging the Inducible Gene Expression Systems With Reporter Genes and Lentiviral Vectors
  • NADPHDiaphorase Staining
  • NNOS Immunofluorescent Staining - 2
  • NNOS Western Blot - 2
  • NO Within the PVN is Involved in Sympatho Excitation in CHF
  • Nonviral Vectors
  • Notes - 2 3 4
  • Overexpression of nNOS by the Adenovirus Vector
  • Overview - 2
  • Packaging and Transfer Vectors
  • Packaging of pAdvheNOS in HEK 293 Cells
  • PET17b Subcloning Vector and pShuttle Cloning Vector
  • Protein Purification
  • Radiotelemetry Monitoring of Blood Pressure and Heart Rate in Conscious Rats
  • Rapid Adenoviral Titer
  • References
  • Regulation of Gene Expression
  • Reporter Genes and Transgene Configurations for Image Analysis
  • Results
  • Sample Preparations
  • Sdspage and Immunoblotting
  • Staining Procedure
  • Subcloning
  • Summary - 2 3
  • Targets for Cardiac Gene Transfer 61 Vascular
  • TATProtein Transduction into Langendorff Perfused Hearts
  • Technique 1 Selective Coronary Gene Delivery
  • Technique 2 Gene Delivery During Cardiopulmonary Bypass - 2
  • The lacZ Reporter Gene
  • Transcardial Perfusion
  • Transient Transfection
  • Xiuqing Wang Robert Cade and Zhongjie Sun Summary
  • Double Luciferase Plasmid

    Double Luciferase Plasmid

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