- Acknowledgments
- Amplification Method The Double Plasmid System
- Animal Models
- Cell Culture
- Cloning
- Congenital Heart Disease
- CreER and CrePRInducible Systems
- CreoxP in the Heart
- Delivery of the AdnNOS Vector or ODNs Into the PVN by Microinjection
- Delivery of the AdnNOS Vector or ODNs Into the PVN by Microinjection see Note
- Determination of nNOS Expression Within the PVN
- Discussion
- Dual Modality Reporter Imaging Technology
- E coli Transformation and Selection
- End Point Dilution Assay
- Expression of AdvheNOS in HAAE1 Cells
- Fluorescent Protein Reporter Gene
- Functional Study Effect of Manipulation of nNOS Within the PVN on Sympathetic Nerve Activity Blood Pressure and Heart Rate
- HeNOS Activity Assay
- Human eNOS Full Length cDNA
- Inducible Gene Expression Systems 31 Introduction
- Info
- Inhibition of nNOS by Antisense
- Introduction - 2 3 4 5 6
- Mark Jones and Walter J Koch Summary
- Lentivirus Vector Gene Delivery Systems
- Luciferase Reporter Gene and Bioluminescence Imaging
- Materials - 2 3
- Measurement of NO Production and heNOS Activity
- Measurement of NOx
- Merging the Inducible Gene Expression Systems With Reporter Genes and Lentiviral Vectors
- NADPHDiaphorase Staining
- NNOS Immunofluorescent Staining - 2
- NNOS Western Blot - 2
- NO Within the PVN is Involved in Sympatho Excitation in CHF
- Nonviral Vectors
- Notes - 2 3 4
- Overexpression of nNOS by the Adenovirus Vector
- Overview - 2
- Packaging and Transfer Vectors
- Packaging of pAdvheNOS in HEK 293 Cells
- PET17b Subcloning Vector and pShuttle Cloning Vector
- Protein Purification
- Radiotelemetry Monitoring of Blood Pressure and Heart Rate in Conscious Rats
- Rapid Adenoviral Titer
- References
- Regulation of Gene Expression
- Reporter Genes and Transgene Configurations for Image Analysis
- Results
- Sample Preparations
- Sdspage and Immunoblotting
- Staining Procedure
- Subcloning
- Summary - 2 3
- Targets for Cardiac Gene Transfer 61 Vascular
- TATProtein Transduction into Langendorff Perfused Hearts
- Technique 1 Selective Coronary Gene Delivery
- Technique 2 Gene Delivery During Cardiopulmonary Bypass - 2
- The lacZ Reporter Gene
- Transcardial Perfusion
- Transient Transfection
- Xiuqing Wang Robert Cade and Zhongjie Sun Summary
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