Antisense DNA oligomer treatment blocking oncogene expression

♦ Short (10-50 base), synthetic, nucleotide sequence

♦ Complementary to specific DNA or RNA sequences

♦ Enter all cells easily

♦ Specific to target individual oncogenes

♦ May produce downregulation

♦ Can inhibit transcription/translation of single gene

♦ Inhibition can be caused by triggering RNase H —degradation of target RNA

—interferes with processing of pre-mRNA

♦ Examples include—pancreatic carcinoma (HRAS)

—prostate cancer cell lines (Block bcl-2 expression)

—poor specificity

—may be increased cell invasiveness

♦ Initial trial results encouraging in AML and lymphoma patients

0 0

Post a comment