E

Fig. 2. This figure illustrates the finding that although mechanoporation can occur, some neurons reseal their perturbated membranes with increased post-traumatic survival. Here confocal images of pre-injury infused dextrans (A), post-injury-infused dextrans (B) and their overlay (C) demonstrate some cortical neurons flooding with the pre-injury infused dextran alone without concomitant flooding with the post-injury administrated tracer (arrows), all of which is suggestive of cell membrane closure/recovery. Note that one double-flooded neuron demonstrating severe damage (arrowhead) and one double-labeled neuron demonstrating less severe pathology (double arrowhead) are also shown. Scale bar: 50 mm. Panel D illustrates a routine fluorescent image that reveals a single labeled cell. In panel E, the same neuron is visualized through the use of antibodies to the fluorophore and then carried to the EM level (F-G). Note that neurons flooding with the pre-injury dextran alone do not show overt pathological damage. Immunoreactive products (anti-Alexa Fluor IR) are labeled with arrows in panel G, which is an enlargement of the area, blocked out in panel F. Scale bar 2 mm. (Adapted with permission from Farkas et al. (2006); Copyright 2006 by the Society for Neuroscience).

Fig. 2. This figure illustrates the finding that although mechanoporation can occur, some neurons reseal their perturbated membranes with increased post-traumatic survival. Here confocal images of pre-injury infused dextrans (A), post-injury-infused dextrans (B) and their overlay (C) demonstrate some cortical neurons flooding with the pre-injury infused dextran alone without concomitant flooding with the post-injury administrated tracer (arrows), all of which is suggestive of cell membrane closure/recovery. Note that one double-flooded neuron demonstrating severe damage (arrowhead) and one double-labeled neuron demonstrating less severe pathology (double arrowhead) are also shown. Scale bar: 50 mm. Panel D illustrates a routine fluorescent image that reveals a single labeled cell. In panel E, the same neuron is visualized through the use of antibodies to the fluorophore and then carried to the EM level (F-G). Note that neurons flooding with the pre-injury dextran alone do not show overt pathological damage. Immunoreactive products (anti-Alexa Fluor IR) are labeled with arrows in panel G, which is an enlargement of the area, blocked out in panel F. Scale bar 2 mm. (Adapted with permission from Farkas et al. (2006); Copyright 2006 by the Society for Neuroscience).

the fact that these reactive axonal changes were found in scattered axons related to other intact axons and their vascular elements, this precluded the potential for direct mechanical renting, suggesting that more subtle intraaxonal changes were at work in the pathogenesis of this progressive axonal change leading to disconnection.

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