Summary

Hematopoietic stem cell investigation has been facilitated by the development of in vitro and in vivo assays of hematopoiet-

Stem cells 39

Fig. 3.5 Competitive repopulation assay

Ly5.2 test cells

Ly5.1 whole bone marrow competitor cells

Lethal irradiation with 9-10Gy

Lethal irradiation with 9-10Gy

Ly5.2 test cells

>16 weeks 104

Ly5.1 mouse

Assess percentage of multilineage bone marrow engraftment using flow cytometry with antibodies directed against Ly5.1 and Ly5.2

>16 weeks 104

Ly5.1 mouse

103 102

ic cell function followed by the identification of molecular cell surface markers that permit the isolation of purified subsets of cells with defined characteristics. Studies in this field have contributed greatly to the understanding of both general stem cell biology and hematopoiesis. Further investigation of cell-intrinsic and cell-extrinsic regulators of hematopoiesis will enable rational manipulation of HSCs and thereby extend the current uses of stem cells in clinical practice.

Lineage commitment

Morrison SJ, Hemmati HD, Wandycz AM et al. (1995) The purification and characterization of fetal liver hematopoietic stem cells. Proceedings of the National Academy of Sciences of the United States of America, 92, 10302-10306.

Nakahata T, Ogawa M. (1982) Clonal origin of murine hemopoietic colonies with apparent restriction to granulocyte-macrophage-megakaryocyte (GMM) differentiation. Journal of Cellular Physiology, 111, 239-246.

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