Conclusions

In this work, the seventh order system is used to describe the fed-batch culture of hybridoma cells and the GA is used to maximize the final MAb production. Optimal feed rate trajectories for a feed stream containing both glucose and glutamine (single-feed case), and separate feed streams of glucose and glu-tamine (multi-feed case) are searched for using the GA. As compared to the optimal constant feed rate, optimal varying feed rate trajectories are shown to improve the final MAb concentration by 10% for the single-feed rate case and by 39% for the multi-feed rate case. In comparison with the DP method, the GA-calculated feed trajectories increase the final MAb by 5%, 6% and 24% for constant-, single- and multi-feed case, respectively.

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