T

probe with radiolabeled DNA from site of potential origin Of replication

RE i

sttcünd dimension -

bubble-arc sttcünd dimension -

BOX 8-3 FIGURE 2 DNA that fell) the process of replication has an unusual structure. Results of restriction enzyme cleavage of DNA in the process of replication are shown. The illustration shows the growth of a "replication bubble" (created by two replication forks progressing away from an origin of replication). The consequences of cutting these replication intermediates is followed by detection by hybridization with the indicated labeled DNA probe. If the red restriction enzyme is used and only the fragments that hybridize to the red DMA probe are examined, the pattern on the left side will be generated, If the blue restriction enzyme and the blue DNA probe is used to detect the resulting OKA fragments, the pattern on the right will be observed. Note that the left-hand pattern starts with a DNA fragment containing a "bubble" and eventually ends with "Y-shaped" molecules The right-hand pattern never has a "bubble" but does assume a full vaiiety of "Y-shaped" intermediates Only a DNA fragment containing an ongm of replication can produce the pattern on the left

origin ™

Was this article helpful?

0 0

Post a comment