Recombinant veterinary vaccines

Amongst the limited number of biopharmaceuticals approved for animal use, recombinant vaccines represent the single largest subgroup. Several such products target pigs, including 'Porcilis pesti' and 'Bayovac CSF E2'. Porcilis pesti, for example, contains a recombinant form of the classical swine fever virus E2 antigen, the immunodominant surface antigen associated with this viral pathogen. It is used to immunize young pigs. An overview of its manufacture is presented in Figure 13.14. The process is initiated by growth of Spodoptera frugiperda cells, typically in a 500 l fermenter. The cells are then infected with the recombinant baculovirus vector, resulting in high-level expression of the recombinant E2 antigen. The antigen is harvested from the production medium by low-speed centrifugation and membrane filtration steps, which serve to remove intact

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Figure 13.14 Overview of the manufacture of the veterinary vaccine 'Porcilis pesti'. Refer to text for specific details

cells/cellular debris. The antigen-containing supernatant is then treated with P-propiolacetone in order to inactivate viral particles present. The antigen is not subjected to subsequent high-resolution chromatographic purification steps, and hence is not purified to homogeneity. The product is then formulated as an oil-in-water emulsion.

Supplements For Diabetics

Supplements For Diabetics

All you need is a proper diet of fresh fruits and vegetables and get plenty of exercise and you'll be fine. Ever heard those words from your doctor? If that's all heshe recommends then you're missing out an important ingredient for health that he's not telling you. Fact is that you can adhere to the strictest diet, watch everything you eat and get the exercise of amarathon runner and still come down with diabetic complications. Diet, exercise and standard drug treatments simply aren't enough to help keep your diabetes under control.

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