Initial product recovery

The initial step of any downstream processing procedure involves recovery of the protein from its source. The complexity of this step depends largely upon whether the product is intracellular or extracellular (Figure 6.1). In general, animal cell-culture-derived biopharmaceuticals are secreted into the media (i.e. are produced as extracellular proteins), whereas the product accumulates intra-cellularly in many recombinant prokaryotic producer cell types. In both cases, upstream processing is followed by initial collection (harvest) of the cells. This is normally achieved by centrifugation, or sometimes microfiltration. If the product is an extracellular one, then the cell paste is generally inactivated (e.g. by autoclaving) and discarded, whereas the product-containing extracellular fluid is subject to further processing. In the case of intracellular product, cell recovery is followed by cellular disruption, in order to release the intracellular contents, including the protein of interest.

Diabetes 2

Diabetes 2

Diabetes is a disease that affects the way your body uses food. Normally, your body converts sugars, starches and other foods into a form of sugar called glucose. Your body uses glucose for fuel. The cells receive the glucose through the bloodstream. They then use insulin a hormone made by the pancreas to absorb the glucose, convert it into energy, and either use it or store it for later use. Learn more...

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