Many methods are available to characterize an allergen. Many of these, such as the determination of protein content, molecular weight, and isoelectric point, are not unique to the study of allergenic compounds. These are simply methods of describing any protein. Several categories of tests, however, are restricted to studying molecules responsible for IgE-mediated symptoms. Both immunologic in vitro methods, such as RAST and Western blotting, as well as in vivo biologic assays, such as end-point dilution skin tests, will be considered here.
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